Introducing Fluidity One

In solution sizing and quantification of native protein in 6 minutes

  • Measure with confidence

    Samples are analysed in solution under near-native conditions, providing the most accurate method for characterising proteins.

  • Native proteins

    Measurement is performed label free, eliminating the risk that a label could affect the property or behaviour of your protein.

  • Built for protein

    A detection range of 0.3 to 30nm (0.1 KDa to 27 MDa) allows you to characterise any protein or protein complex.

  • Take your sample further

    Requiring just 5 µl of sample, the Fluidity One allows you to run more experiments and collect more data.

  • Reliable data

    Disposable chips eliminate risk of contamination and mean there’s no need for time-consuming wash cycles.

  • Get results quickly

    Run times as fast as 6 minutes save you time and allow you to test more conditions.

Applications

A growing range of applications using Microfluidic Diffusional Sizing

In solution protein sizing

Knowledge of a protein’s size can provide insights on how it is folded, whether it has been expressed in a full length or truncated form, if it is in an active or inactive conformation, whether it is monomeric or oligomeric or indeed whether it is in complex with additional proteins or biomolecules.

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Protein quality analysis

Poor quality protein samples can result in low quality data and wasted time in downstream applications. The speed, low sample requirement and minimal sample prep required by the Fluidity One make it the perfect tool for routine protein prep quality analysis.

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Study of protein aggregation

The study of the mechanisms of protein aggregation is of central importance to understanding fundamental biological processes such as protein assembly and turnover, as well as pathologies that are characterised by the presence of protein aggregates - such as Parkinson’s and Alzheimer’s disease. With the Fluidity One you can assess the formation of aggregates directly, without the use of labels.

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Monitoring protein-protein interactions in solution

Understanding of a protein’s function is in many cases gained through an awareness of what it interacts with, with what affinity and at what time. Monitoring protein-protein interactions (PPIs) therefore can provide deep insights into everything from basic biology to the mechanisms of disease. The Fluidity One can provide insights into PPIs in solution.

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Do you have an application in mind?

If you think our technology could advance your research we'd love to hear how.

How does the Fluidity One work?

The rate of diffusion of a particle is inversely proportional to its hydrodynamic radius – a relationship that is described by the Stokes-Einstein equation. The Fluidity One exploits this by measuring the rate of diffusion of proteins under steady state laminar flow in a microfluidic system.

To achieve steady state laminar flow, the Fluidity One uses a microfluidic system in which micro-litre volumes do not mix convectively. Consequently when a protein sample and analyte solution are run as adjacent (non-mixing) streams, the only way protein can migrate into the analyte stream is by diffusion – at a rate that is inversely proportionate to its size. This is the measurement step in the Fluidity One system, and occurs without labelling in the diffusion chamber of the chips.

Following measurement, the streams are split and a latent, fluorogenic dye is introduced to label the protein and enable detection. Fluorescence intensity in both streams is then measured, the total of which is used to determine concentration of protein, and the ratio of which can be used to determine the diffusion coefficient, and consequently the hydrodynamic radius.

*sample dependent **predicted 

FAQ

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Fluidic Analytics is changing how proteins are characterised

We're doing this with novel, cutting edge technology and a fantastic, committed team

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