Latent analysis of unmodified biomolecules and their complexes in solution with attomole detection sensitivity

Emma V. Yates, Thomas Müller, Luke Rajah, Erwin J. De Genst, Paolo Arosio, Sara Linse, Michele Vendruscolo, Christopher M. Dobson, Tuomas P.J. Knowles.

Nature Chemistry 7(10):802-9, 2015.

In this paper, Yates et al show that the technology behind Fluidic Analytics instruments - microfluidic diffusional sizing - can be used to accurately size a range of proteins. Results were compared to those obtained using Pulsed Field Gradient NMR and Analytical Ultracentrifugation and found to agree closely (Figure 1).


Figure 1: Rh values determined in this manner are compared to those obtained with PFG-NMR (lilac) and AUC (aqua). The studied molecules vary by over three orders of magnitude in molecular weight and include intrinsically disordered proteins and heterogeneous mixtures.

Finally, they demonstrate that Microfluidic Diffusional Sizing can be used to characterise protein interactions without the use of tags, using the platform to explore the binding of α-synuclein to a nanobody, NbSyn1 (Figure 2)

novel Parkinson's-related immune complex

Figure 2: Characterisation of a novel Parkinson’s-related immune complex between a Nanobody and equimolar (5 μM) α-synuclein.

Read the complete paper here